An A_2AAR agonist decreases NF-κB nuclear translocation and NET-induced proinflammatory cytokine production in macrophages. Control macrophages were incubated with NETs in the presence or absence of A_2AAR agonist. (A) Translocation of NF-κB (in red) was impaired in cells treated A_2AAR agonist. Nuclei were stained in blue. Scale bar, 10 µm. (B) Translocation of NF-κB was quantified and graphed as a percentage of total cells. (C) Gene expression analysis shows A_2AAR agonist significantly decreased NET-induced proinflammatory cytokine in macrophages. *P < .05, **P < .01, ***P < .001, ****P < .0001, Mann-Whitney U test. Results are the means ± SEM of n = 4-5.