GSK3β localized to centrosomes. (A) Immunofluorescence image showing GSK3β is localized to the nucleus and centrosome pairs in interphase Jeko cells. (B) Enlarged image of the boxed area in panel A. Centrosome pairs are marked by arrows. (C-F) Single- or multichannel images of coimmunostaining of GSK3β and pericentrin in wild-type Ly-1 cells showing their colocalization to the centrosomes. (G-J) Single- or multichannel images of coimmunostaining of GSK3β and pericentrin in GSK3β-null Ly-1 cells showing that staining is specific to GSK3β. (K) Immunofluorescence image showing GSK3β (green) localized to firework-like structures resembling mitotic spindles in mitotic Jeko cells. (L) Enlarged image of the mitotic cell shown in panel K. (M) Overlay image showing the microtubule structure by α-tubulin (red) staining and DNA (blue). (N) Enlarged image of the boxed area in panel M. (O-P) Images showing that spindle structure staining of GSK3β is absent in GSK3β-null Ly-1 cells. (Q) Immunofluorescence image showing GSK3β localized to mitotic spindle structure and polarized centrosomes in 9-ING-41–treated Jeko cells. (R) Enlarged image of the representative mitotic cell shown in panel Q. Original magnification ×600.