Targeting of the Phf6 gene in the hematopoietic system. (A) Gene-targeting strategy for the Phf6 gene. (B) RT-qPCR analysis of Phf6 in CD150⁺CD34⁻LSK HSCs and CD150⁺CD34⁺LSK multipotent progenitors. Hprt1 was used to normalize the amount of input RNA. Data are shown as the mean plus or minus standard error of the mean (SEM) (n = 3). The significance of differences relative to WT is shown. *P < .05, **P < .01, ***P < .001 by the Student t test. (C) Western blot analysis of the spleen and thymus. Vinculin was used as a loading control. (D) Total BM cell numbers of the bilateral pairs of femurs and tibiae at 12 weeks of age.