Most mRNAs bound by miR-144/451-Ago are not repressed. (A) Overlap between mRNAs with altered stability and/or Ago binding in KO erythroblasts. (B) Cumulative distribution function (CDF) plot showing fold change in mRNA abundance between KO and WT FL erythroblasts for 1243 mRNAs with KO-depleted HITS-CLIP peaks and 1879 mRNAs with nondepleted peaks. (C) Nucleotide complementarity patterns between miRNA seed sequences and their target mRNAs. (D) Effects of peak location in CDS vs 3′ UTR on mRNAs with KO-depleted peaks containing canonical miR-451 seed matches. (E) Effects of canonical vs 6-mer and 6-mer-offset miR-451 seed matches on KO-depleted peaks mapping to 3′ UTRs. (F) Effects of peak location (CDS vs 3′ UTR) on mRNAs with KO-depleted peaks containing canonical miR-144-3p seed matches. (G) Effects of canonical vs 6-mer and 6-mer-offset miR-144-3p seed matches on KO-depleted peaks mapping to 3′ UTRs. *P < .05, **P < .01, ***P < .001, †P < 10−6 by the Kolmogorov-Smirnov test. All other curves are not significantly different compared with all genes.