miR-144/451 recruits Ago to thousands of erythroid mRNAs. (A) Genomic locations of 6651 Ago HITS-CLIP peaks for all miRNAs. (B) Fold change (x-axis) of Ago HITS-CLIP peak signals in miR-144/451 KO vs WT erythroblasts. The dotted line indicates more than threefold depletion in KO erythroblasts at FDR ≤ 0.05. The top volcano plot highlights KO-depleted HITS-CLIP peaks in black and 3 established miR-451 targets in color. The middle panel highlights peaks containing canonical seed matches to miR-144 or miR-451. The bottom panel highlights peaks containing canonical seed matches to 3 abundant miRNAs that are expressed normally in KO cells. (C) The number of peaks containing canonical seeds for the miRNAs depicted in panel B with the percentage depleted in KO erythroblasts indicated above each bar. *P < .0001 by the χ² test. Misc., miscellaneous.