CEBPE binds the +6-kb enhancer and autoregulates its expression. (A) ChIP-seq track depicting occupancy of CEBPE at the +6-kb enhancer in murine bone marrow cells.³⁷  (B) ChIP-qPCR shows enriched binding of CEBPE at the +6-kb enhancer. Three primer pairs were used for PCR analysis. Luciferase reporter assay (C) and EMSA (D) were performed with lysates from CEBPE-expressing cells with target and mutant oligonucleotides (oligos) as described for Figure 4E and 4F. (E) Immunoblot for CEBPE expression in 32D cells stably transduced with lentivirus expressing murine Cebpe (Cebpe O/E). α-Tubulin was used as an endogenous control. (F) qRT-PCR for Cebpe expression in control and Cebpe O/E 32D cells (left panel). Two primer pairs were used: exonic primers that detect total Cebpe transcript levels and 3′-UTR–specific primers that exclusively detect endogenous Cebpe transcripts. qRT-PCR for Ltf and Ngp expression in control and Cebpe O/E 32D cells (right panel). Y-axis represents relative expression of genes normalized to Gapdh. ****P < .0001.