Eosinophil-specific deletion of Trib1 results in Ly6G⁺ eosinophil development with improved preservation of EoPs. (A) Representative plots of BM EoP, gated on live, lineage⁻CD34⁺ cells from Trib1^+/+ and Trib1^ΔEos mice. Frequency of live cells (B) and absolute numbers (C) of BM EoP from Trib1^+/+ and Trib1^ΔEos mice; n = 6 mice per group, pooled from 2 experiments. (D) Representative plots of BM eosinophils from Trib1^+/+ and Trib1^ΔEos mice gated on live, CD11b⁺ cells. Frequency of live cells (E) and absolute numbers (F) of BM eosinophils by CCR3 expression. (G) Representative histogram of Ly6G expression, gated on live, CD11b⁺SiglecF⁺CCR3⁺, Trib1^+/+ (blue), Trib1^ΔHSC (red). (H) Frequency of SiglecF⁺CCR3⁺ cells expressing Ly6G; n = 11 mice per group, pooled from 4 experiments. (I) Representative plots of BM neutrophils from Trib1^+/+ and Trib1^ΔEos mice, gated on live, SiglecF⁻ cells. (J) Frequency of live cells and (K) absolute numbers of BM neutrophils; n = 11 mice per group, pooled from 4 experiments. *P = .0289; ****P < .0001; unpaired Student t test. Frequencies and error bars are mean ± SEM of live cells.