Analysis of pooled clones over time reveals instability in the clonal composition of PB during native hematopoiesis. (A) Visualization of the distribution of GFP, YFP, RFP, and CFP in the PB of representative Conf-E2a^Cre, Conf-VE^Cre, Conf-Flk1^Cre, and Conf-Vav1^Cre mice at 2, 7, 12, 16, 20, 24, and 26 months of age. (B) Fold change in the percentage of GFP relative to the preceding time point. Each line represents an independent mouse. Mice from all 4 cohorts are shown: Conf-E2a^Cre (n = 9), Conf-VE^Cre (n = 7), Conf-Flk1^Cre (n = 6), and Conf-Vav1^Cre (n = 10). Evolution of percentage of GFP for individual PB lineages is shown in supplemental Figure 2 for each mouse, each mouse strain, and without normalization. Source data are provided in supplemental Table 1.