Dominant-negative NFE2 mutations confer an advantage in cytokine-dependent proliferation and show increased repopulation capacity ex vivo. (A) Ba/F3 cells were transduced with pLeGO-iG constructs, as indicated, and FACS sorted for GFP. Cells were grown in the absence or presence of IL-3. (B) Ba/F3 cells transduced and sorted for GFP-positive Jak2^V617F (iG) cells were transduced with pLeGO-iC2 constructs, as indicated, and FACS sorted for mCherry. (A-B) Experiments were performed using 2 independent infections and sorts per construct, followed by n = 3 assays carried out in duplicates each. (C) 5-FU treated C57/B6 BM was transduced with pLeGO-iG constructs, as indicated, followed by FACS sorting for GFP-positive cells into methylcellulose. Colonies were stained with benzidine before counting. (D) C57/B6 BM was transduced with pLeGO-iG constructs, as indicated, followed by FACS sorting for GFP-positive cells into methylcellulose. Colonies were counted every 7 to 9 days, followed by replating of 20 000 cells. n = 3-4 in duplicates each. Data were analyzed for statistical significance by 1-way ANOVA with Bonferroni’s post hoc multiple comparison test. *P < .05; **P < .01; ***P < .001; **** P < .0001. WT, wild-type.