Loss of PHF6 synergizes with ectopic TLX3 expression to drive leukemogenesis. (A) Kaplan-Meier survival curve of host mice transplanted with Phf6-deleted or Phf6 control cells expressing MSCV-empty-GFP (empty-GFP) or MSCV-Tlx3-GFP (Tlx3-GFP) retrovirus. n = 11 Phf6-control;Tlx3-GFP, n = 10 Phf6^lox/Y;Tie2-cre^Tg/+;Tlx3-GFP, n = 6 Phf6-control;empty-GFP, and n = 6 Phf6^lox/Y;Tie2-cre^Tg/+;empty-GFP from 4 donors per genotype. Data were analyzed using the Gehan–Breslow–Wilcoxon test. (B) Cell surface phenotype of Tlx3-GFP tumors of indicated Phf6 genotype with comparison with wild-type spleen cells, showing expression of CD19 and B220. (C) Plots showing no GFP expression in normal B cells of a wild-type spleen and GFP⁺CD19⁺B220^neg cells in Tlx3-GFP transplants. (B-C) The phenotype and quantification ± standard error of the mean are representative of all analyzed Tlx3-GFP tumors (n = 3 Phf6-control;Tlx3-GFP, n = 10 Phf6^lox/Y;Tie2-cre^Tg/+;Tlx3-GFP).