Low vitamin D diet prevents myelofibrosis and osteosclerosis in the basic model. (A) H&E and silver staining in femur sections of VDR^+/+ and VDR^−/− recipient mice transplanted with VDR^+/+ BM (1 month after transplantation). Recipient mice were fed with a normal or low vitamin D diet after weaning, and transplanted at 7 to 8 weeks old. Scale bars, 500 μm (black) and 50 μm (white). (B) Survival, donor chimerism, and blood cell counts in VDR^+/+ and VDR^−/− recipient mice (low vitamin D diet) transplanted with VDR^+/+ BM. Combined data of at least 3 independent experiments are shown. Data are represented as mean plus or minus SEM. *P < .05, **P < .01 (Kaplan-Meier analysis and Student t test).