TAK-243 activates stress-response pathways. (A) The impact of UAE inhibition on selected components of the stress-response pathway is shown by western blotting of extracts from MM1.S and U266 myeloma cells, with bortezomib serving as a positive control. Loading was confirmed using either β-actin or histone H3 as an internal control. (B) qPCR was also performed to evaluate any changes in expression of stress-response pathway genes under the same conditions.