Maea deletion impairs BM macrophage development. (A) Representative FACS plots of the BMNC gating strategy for each cell population shown in panel B. (B) Representative histograms showing Maea expression on BM leukocytes from control (Ctrl) littermates and Maea^Csf1r-Cre mice. (C) Deletion efficiency of Maea by Csf1r-Cre on BM macrophages (Mϕ) as determined by FACS (n = 8). (D) Quantification of total BM cellularity in control and Maea^Csf1r-Cre mice (n = 8). (E) Quantification showing significant reduction of macrophages in the BM of Maea^Csf1r-Cre mice compared with littermate controls (n = 8). Data are shown as mean plus or minus SEM. **P < .01, ***P < .001, ****P < .0001 by unpaired Student t test. FSC, forward scatter; MFI, mean fluorescence intensity; SSC, side scatter.