Infusion study for kinetics and incorporation into thrombus formation under flow conditions. (A) Schema of the human platelet infusion study using thrombocytopenic NSG mice. (B) The presence of human platelets, as assessed by CD42b⁺/anucleate platelet-sized cells, in mouse blood after the infusion of ASCL-PLTs or platelet concentrates. (C) Ex vivo thrombus formation under flow conditions, observed under a fluorescence microscope. The blood samples were taken from thrombocytopenic NSG mice infused with ASCL-PLTs or platelet concentrates. Scale bar, 50 μm.