Differentiation of the ASCL into ASCL-MKs. (A) Representative data for CD41/CD42b expression in ASCL-MKs (n = 3). (B) Representative data for DNA ploidy in ASCL-MKs. (C) Representative data for c-MPL surface expression in the ASCL and TPO secretion levels during MK differentiation from the ASCL (n = 2). *P < .01. (D) Expression of serotonin, VWF, and PF4 in MKs derived from the ASCL cultured in MKLI media for 8 days. Cells stained with the antibody for CD42b (red), for DAPI (blue), and for serotonin, VWF, or PF4 (green). Scale bars, 10 μm. (E) Electron microscopic images of ASCL-MKs. Scale bars, 5 μm. DMS, demarcation membrane system; G, granule; M, mitochondrion; N, nucleus.