Manufacturing platelets from human adipose–derived mesenchymal stem/stromal cells. Human subcutaneous adipose tissue was used to generate adipose-derived mesenchymal stromal/stem cell line (ASCL) that fully satisfies the criteria for defining mesenchymal stem cells as determined by The International Society for Cellular Therapy. When cultured in Megakaryocyte (MK) Lineage Induction Media, ASCL produces and secretes thrombopoietin (TPO), which binds to c-Mpl, supporting MK differentiation. Once mature, MKs can be cultured within a cell expansion system and generate functional human ASCL-Platelets (PLTs).