HDAC6 deficiency fails to reduce tumor burden of MPL^W515L mice. (A) Schematic showing experiment design. Congenic WT recipient mice received MPL^W515L-transduced Hdac6^−/− or Hdac6^+/+ BM. WT recipients transplanted with MPL^WT-transduced BM served as controls. (B) WBC, (C) platelet (PLT) count, (D) RBC counts, (E) hemoglobin (Hgb), and (F) hematocrit (HCT) levels of the indicated transplanted recipient mice were determined on day 18 (the first 2 columns in MPL^W515L) and day 25 (the last 2 columns in MPL^W515L). (G-H) Mice were euthanized and spleen weights were measured on day 25. (I) BM cells isolated from 2 femurs and 2 tibias from the indicated transplanted recipient mice were counted. Statistical analysis was performed by using multiple two-tailed unpaired Student t test. ***P < .001; **P < .01; *P < .05.