Wdr26 is upregulated during terminal erythropoiesis. (A) Heat maps showing the mRNA sequencing results of Wdr26 and control genes in MEL cells before and after DMSO-induced erythroid-like differentiation (left) and in R2-R5 subpopulations of primary mouse erythroblast²³  (right, MPFE). MEL, mouse erythroleukemia cells; MPFE, mouse primary fetal liver erythroblasts. (B) Heat map for WDR26 and control genes in terminally differentiating human erythroblasts.²⁴  Stages of erythroblasts shown include proerythroblast (ProE), early (EBaso) and late (LBaso) basophilic erythroblast, polychromatic erythroblast (Poly), and orthochromatic erythroblast (Ortho). (C-D) Quantitative reverse-transcription-PCR analyses of Wdr26 in differentiating MEL cells (C) and primary mouse erythroblasts isolated from E14.5 mouse fetal liver cells (D). Error bars represent SEM from 3 replicates. *P < .05, **P < .01. (E) Western analysis of Wdr26 in multiple mouse tissues. (F) Wdr26 mRNA expression in different hematopoietic lineages isolated from adult mouse bone marrow. Error bars represent SEM from 3 replicates. **P < .01. (G-H) Western analysis of Wdr26 in (G) primary mouse erythroblasts and (H) differentiating MEL cells. SEM, standard error of the mean.