Hyperinflammatory phenotype and altered leukocyte counts are absent in Shp1;Gp1ba-Cre mice. (A) Protein levels of Shp1 and GAPDH in platelet lysates of the indicated genotypes were determined by capillary-based immunoassays with the respective antibodies. Platelet Shp1 protein levels were determined by normalizing Shp1 peak areas by GAPDH peak areas (Shp1/GAPDH ratio), n = 6 mice per genotype. (B) Mice were monitored every week for signs of motheaten-like phenotype (paw, nose, or ear inflammation). Percentage of disease-free mice for the indicated period was calculated using Kaplan-Meier survival analysis with a log-rank (Mantel-Cox) test. ***P < .001, n values are indicated in the figure. (C) Representative photographs of inflamed paws in Shp1;Pf4-Cre mice. (D) Monocyte, atypical lymphocyte, and large immature cell counts, n = 11 to 58 mice per genotype. (E) Spleen/body weight ratio, total splenocyte counts, granulocyte (Gr-1^hiF4/80⁻CD11c⁻B220⁻) counts; cDC (CD11c^hiB220⁻) counts, and activated CD86⁺ cDC MFI of the indicated genotypes, n = 7 to 8 mice per genotype. (A,D-E) Asterisks refer to significant difference in Shp1^fl/fl;Gp1ba-Cre^+/KI or Shp1^fl/fl;Pf4-Cre^+/KI mice compared with Shp1^+/+;Gp1ba-Cre^+/KI or Shp1^+/+;Pf4-Cre^+/KI mice, respectively (***P < .001; 1-way ANOVA with Sidak’s test); data represent mean ± SD.