Gp1ba-Cre-mediated and Pf4-Cre-mediated recombination of blood, bone marrow, and spleen cells. (A) Flow cytometry analysis of peripheral blood cells showing EGFP and tdTomato fluorescence of platelets (CD41⁺), n = 6 mice per genotype; erythrocytes (TER-119⁺CD41⁻), n = 6 mice per genotype; and leukocytes (CD45⁺CD41⁻), n = 5 to 7 mice per genotype from mT/mG^+/fl, mT/mG^+/fl;Gp1ba-Cre^+/KI, and mT/mG^+/fl;Pf4-Cre^+/KI mice. (B) Flow cytometry analysis of bone marrow cells showing EGFP and tdTomato fluorescence of CD41^hiTER-119⁻ bone marrow cells, n = 4 to 5 mice per genotype; GPIbα^hiTER-119⁻ bone marrow cells, n = 4 to 5 mice per genotype; and TER-119⁺GPIbα^- bone marrow cells, n = 3 mice per genotype from mT/mG^+/fl, mT/mG^+/fl;Gp1ba-Cre^+/KI, and mT/mG^+/fl;Pf4-Cre^+/KI mice. (C) Flow cytometry analysis showing EGFP and tdTomato fluorescence of the indicated spleen cell populations, n = 5 mice per genotype from mT/mG^+/fl, mT/mG^+/fl;Gp1ba-Cre^+/KI, and mT/mG^+/fl;Pf4-Cre^+/KI mice. (A-C) Numbers in gates represent mean percentages of respective cell populations. Mean ± SD are shown in supplemental Table 4. Flow cytometry gating strategies for peripheral blood and bone marrow cells are shown in supplemental Figure 6. Gating strategies for spleen cell populations are shown in supplemental Figure 7.