Phenotypic reversion by preventing release of α-defs. (A) Effect of colchicine and BMT on plasma α-defs. Def⁺⁺ mice were given colchicine or saline in their drinking water for 2 weeks. A second cohort of WT mice underwent BMT from Def⁺⁺ mice that received either colchicine or saline for 2 weeks. Plasma α-Def-1 was measured by ELISA. Results shown are the mean ± SD in 13 mice. (B) Effect of colchicine and BMT on thrombus development in Def⁺⁺ mice. IVC stenosis was induced in Def⁺⁺ mice given colchicine or saline in their drinking water and in Def⁺⁺ and WT mice after BMT as described in panel A. Thrombus weight was measured as in Figure 4. The mean ± SD in 11 mice is shown (* and #, P < .05). (C) Effect of colchicine on IVC structure in Def⁺⁺ mice. Scanning electron microscopy images of the surface (a and c) and interiors (b and d) of thrombi extracted from untreated Def⁺⁺ mice (a and b) and Def⁺⁺ mice given colchicine (c and d) are shown. Scale bars, 30 μm.