RNAseq analysis of splenic B cells and B-cell lymphomas. (A) PCA of RNAseq gene expression data from 1 wt splenic B-cell sample (stimulated or not with LPS), 3 premalignant B-cell samples (stimulated or not with LPS) and 20 tumor samples. Each wt and premalignant B-cell sample is pooled mRNA from 4 different mice. Same mice for LPS-stimulated and unstimulated B cells. Premalignant splenic B-cell samples were from c-myc-KIE_μ, c-myc-KIC_μ, and c-myc-KIC_α mice. (B) Heat map of the 100 most variable gene expressions across all samples; hierarchical clustering of genes per sample according to Pearson’s correlation metric and Ward’s method. Same symbols, colors, and group definitions as in panel A. (C) Heat map of 100 most variable gene expressions across the wt and premalignant B-cell samples only; hierarchical clustering of genes per sample according to Pearson’s correlation metric and Ward’s method. (D) Expression of 4 representative genes (drawn from GO:0002312 “B-cell activation involved in immune response”) in wt and premalignant samples. AICDA, gene for the AID protein implicated in Ig somatic hypermutation and CSR; CDH17, a member of the cadherin superfamily; CCR6, the MIP-3α receptor; EXOSC3, subunit of the RNA exosome.