KIR3DL1 single⁺NK cells in the d-rsKIR group (Bw4Bwx-Bw4Bwx) show higher reactivity compared with other groups (nsKIR, dsKIR, and rsKIR). The percentage of KIR3DL1 single⁺ NK cells at day 180 (A) after transplantation in the d-rsKIR (pairs of donor Bw4Bwx and host Bw4Bwx, n = 55), dsKIR (pairs of donor Bw4Bwx and host Bw6Bw6, n = 18), rsKIR (pairs of donor Bw6Bw6 and host Bw4Bwx, n = 16), and nsKIR groups (pairs of donor Bw6Bw6 and host Bw6Bw6, n = 25). The expression of CD107a (B) and IFN-γ (C) of KIR3DL1 single⁺ NK cells against K562 cells at day 180 after transplantation in the d-rsKIR, dsKIR, and rsKIR groups. MFI expression of DNAM-1 (D) and CD122 (E) on KIR3DL1 single⁺ NK cells at day 180 after transplantation in the d-rsKIR, dsKIR, and rsKIR groups. Heatmap of average NK-cell marker expression on single KIR3DL1⁺ NK cells from each group at different time points. Sample origin color-coding according to the color key in panel F. In order to adjust all the data to the same order of magnitude, we processed the data as follows: the MFI of DNAM-1 and Bcl-2 was divided by 100; the MFI of NKP30, NKP46, and CD122 was divided by 10; and the expression of CD25 was multiplied by 10.