KIR2DL1 single⁺NK cells in the d-rsKIR group (C2Cx-C2Cx) showed higher reactivity compared with other groups (nsKIR, dsKIR, and rsKIR). The percentage of KIR2DL1 single⁺ NK cells at day 90 (A) and day 180 (D) after transplantation in the d-rsKIR (pairs of donor C2Cx and host C2Cx, n = 39), dsKIR (pairs of donor C2Cx and host C1C1, n = 23), rsKIR (pairs of donor C1C1 and host C2Cx, n = 9), and nsKIR groups (pairs of donor C1C1 and host C1C1, n = 43). The expression of CD107a (B,E) and IFN-γ (C,F) of KIR2DL1 single⁺ NK cells against K562 cells at days 90 and 180 after transplantation in the d-rsKIR, dsKIR, and rsKIR groups. MFI expression of DNAM-1 (G-H) and CD122 (I-J) on KIR2DL1 single⁺ NK cells at days 90 and 180 after transplantation in the d-rsKIR, dsKIR, and rsKIR groups. Heatmap of average NK-cell marker expression on single KIR2DL1⁺ NK cells from each group at different time points. Sample origin color-coding according to the color key in panel K. In order to adjust all the data to the same order of magnitude, we processed the data as follows: the MFI of DNAM-1 and Bcl-2 was divided by 100; the MFI of NKP30, NKP46, and CD122 was divided by 10; and the expression of CD25 was multiplied by 10.