HOXA10-AS is highly expressed in HSPCs and KMT2A-rearranged leukemic cells. (A) Schematic of the genomic locus around HOXA10-AS, which is antisense to HOXA10 and miR-196b. (B-C) Mean expression of HOXA10-AS (B) and HOXA10 and miR-196b (C) in defined blood cell populations from healthy individuals as a percentage of the housekeeping (HK) gene β-2 microglobulin or RNU48 and U6 as measured by qRT-PCR (n ≥ 2; presented as mean ± SEM). HSCs, common myeloid progenitor cells (CMP), granulocyte-monocyte progenitor cells (GMP), megakaryocyte-erythroid progenitor cells (MEP), megakaryocytes (Mk), erythroid precursors (Ery), granulocytes (GC), monocytes (Mono), CD4 and CD8 T cells, and natural killer cells (NKC). (D-E) Mean expression of HOXA10-AS (D) and HOXA10 and miR-196b (E) in AML cell lines as a percentage of the housekeeping genes β-2 microglobulin or RNU48 and U6 as measured by qRT-PCR (n ≥ 2; presented as mean ± SEM). On the right, HOXA10-AS, HOXA10, and miR-196b expression in KMT2A-rearranged and non–KMT2A-rearranged cell lines (unpaired Student t test). (F) Normalized expression of HOXA10-AS in cytogenetically defined pediatric patient cohorts (1-way variance analysis). ns, not significant.