Recurrently mutated genes in PMBL. (A) Comutation plot of recurrently mutated CCGs; gene-by-sample matrix color-coded by mutation type (middle); ranked by significance (MutSig2CV³⁸ q < 0.1, right); number and frequency of recurrent mutations (left); total number of mutations (top); allelic fraction and base substitution distribution of mutations in individual samples (below); Asterisks indicate 3 hypermutator cases; ts, transitions; tv, transversions; DNP, dinucleotide polymorphism. (B-C) Spatial clustering of mutations was discovered with CLUMPS⁴¹ ; examples include STAT6 (panel B: PDB: 4y5u; STAT6 dimer is shown with individual molecules in gray and cyan, respectively; DNA in purple) and ZNF217 (panel C: PDB: 2kmk; DNA in cyan). Mutated residues are shown in red and color-intensity and thickness of line scales with number of mutations. (D) Mutation diagrams (lollipop figures) for all significantly mutated genes; aa, amino acid. For each significantly mutated gene (MutSig2CVq < 0.1), all nonsynonymous mutations are visualized within the functional domains of the respective protein using MutationMapper v2.1.0.^85,86  Genes are ranked by significance as in (A).