Vit C–treated CD8⁺iTregs enhance Foxp3 stability during GVHD development. (A) Experimental scheme: lethally irradiated BALB/c mice were adoptively transferred with 5 × 10⁶ Rag1^−/− BM cells and 1 × 10⁶ CD8⁺ iTregs (Ly5.2⁺). Three days later, 2 × 10⁶ CD25-depleted T cells from C57BL/6 (Ly5.1⁺) congenic mice were injected IV. On day 7 after allogeneic BMT, spleen, liver, and mesenteric lymph nodes (mLNs) were harvested and analyzed. (B) Foxp3⁺ retention by transferred control and Vit C–treated CD8⁺ iTregs. (C) IFN-γ expression of transferred CD8⁺ iTregs. Data are combined from 2 independent experiments (n = 7 per group). *P ≤ .05, **P ≤ .01, ***P ≤ .001, ****P ≤ .0001, Student t test.