AMPK KO AML cells markedly downregulate histones that are acetylated on multiple lysine residues. (A-B) Immunoblotting with WT or AMPK KO bulk (GFP⁺) AML cells or the L-GMP population to detect acetylated histones (A) or acetylated p53, α-tubulin, or CBP (B). (C) Principal component analysis of the histone proteoform data set obtained from normal bone marrow (BM) cells, WT AML cells, and AMPK KO AML cells. (D) Acetylation status of individual lysine residues from the histone proteoform analysis, normalized against normal BM cells (n = 3). (E) The abundance of histone proteoforms that were acetylated on 2 lysine residues (n = 3). All data represent mean ± standard deviation. *P < .05; **P < .01; ***P < .001 by 1-way ANOVA.