Effects of a single oral intake of ibrutinib on platelet FcγRIIA stimulation. Three healthy donors (A, B, and C) received ibrutinib, 2 doses of 140 mg each. Blood was drawn just before the intake and then 3 hours, 1 day, 2 days, and 1 week after intake. Blood samples were preincubated for 3 minutes before platelet Fc receptor stimulation by CD32 cross-linking with AT10 and Fab2, or anti-CD9 antibody (see legends for Figures 1 and 2). Platelet aggregation (A); ATP secretion (B). The luminescence signals were calibrated for each blood donor in every experiment by the addition of ATP standard solutions. Values are mean ± SD of triplicate determinations. AB, antibody.