JW480 impedes thrombosis and hemostasis. (A) Adult SD rats were injected IV with vehicle control or 8 to 11 mg/kg JW480 for 3 hours before blood was harvested into acid citrate dextrose. Platelet function was measured in recalcified blood using impedance aggregometry in response to 50 to 100 ng/mL convulxin. Aggregation was normalized to the maximum value observed in the absence of JW480, which was set to 1.0 (*P < .04; n = 3). (B) Adolescent SD rats (3.5 to 5 weeks old) were injected IV with vehicle control or 40 mg/kg JW480 for 5 minutes. Carotid artery thrombosis was induced with 40% FeCl₃ for 2 minutes and scored as the “time to occlusion,” which is defined as reduction of blood flow to 25% of normal levels prior to injection of JW480 (control 10.3 minutes vs JW480 14.8 minutes; *P < .004; n = 10 per group). (C) Adult SD rats (≥6 months) were injected IV with vehicle control or 3.5 to 10 mg/kg JW480 as in panel B before measuring bleeding times from lateral tail veins (control 30 vs JW480 7.8 minutes; *P < .03; n = 6 per group). TTO and bleeding data were expressed as medians and analyzed using Mann-Whitney U tests.