Proinflammatory factors are involved in HBA HC output. (A) Concentration (fg/mL) of TNF-α protein in the supernatant of OP9-DL1-B1 cultures containing E10.5 (34 to 39 sp) HBA EC, MФ, or MIX, as determined by FCAP assay. n = 3; *P = .039; **P = .005. Number of (B) CD45⁺ cells and (C) CFU-C number per 1 ee of HBA cells in EC cultures supplemented with different TNF-α concentrations. n = 4. Number of (D) CD45⁺ cells and (E) CFU-C/ee of E10.5 HBA cells in the OP9-DL1-B1 cocultures containing EC, EC+TNF-α nAb (0.4 μg/mL), EC+TNF-α (10 ng/mL), MIX, MIX+TNF-α nAb (0.4 μg/mL). n = 4. Colony types are indicated by color bars. *P < .05; **P < .01; ***P < .001.