Figure 6.
Genes of proinflammatory pathways are expressed in HBA cells. (A) Relative mRNA expression of IL1α, IL1β, TNFα, and IL-6 normalized to actin in GFP+ (G+) and GFP− (G−) cells in the E10.5 MacGreen HBA as determined by qRT-PCR. OP9-DL1-B1 (DL1) is the negative control. n = 3; *P < .05. (B) Relative mRNA expression of IL1α, IL1β, TNFα, and IL-6 as normalized to actin in macrophages (MΦ, CD45+CD11b+F4/80+Gr1−), ECs (CD31+CD45−) in the E10.5 HBA as determined by qRT-PCR. OP9-DL1-B1 (DL1) is the negative control. n = 4; *P < .05; **P < .01; ****P < .0001. (C) Fold-change in the relative mRNA expression of IL1 and TNF receptor genes (IL1R1, IL1Rap, IL1R2, TNFR1, TNFR2) in E10.5 HBA EC and HCs as compared with MCs. IL1R1, IL1Rap, IL1R2, TNFR2, n = 3; TNFR1, n = 4; *P < .05.

Genes of proinflammatory pathways are expressed in HBA cells. (A) Relative mRNA expression of IL1α, IL1β, TNFα, and IL-6 normalized to actin in GFP+ (G+) and GFP− (G−) cells in the E10.5 MacGreen HBA as determined by qRT-PCR. OP9-DL1-B1 (DL1) is the negative control. n = 3; *P < .05. (B) Relative mRNA expression of IL1α, IL1β, TNFα, and IL-6 as normalized to actin in macrophages (MΦ, CD45+CD11b+F4/80+Gr1−), ECs (CD31+CD45−) in the E10.5 HBA as determined by qRT-PCR. OP9-DL1-B1 (DL1) is the negative control. n = 4; *P < .05; **P < .01; ****P < .0001. (C) Fold-change in the relative mRNA expression of IL1 and TNF receptor genes (IL1R1, IL1Rap, IL1R2, TNFR1, TNFR2) in E10.5 HBA EC and HCs as compared with MCs. IL1R1, IL1Rap, IL1R2, TNFR2, n = 3; TNFR1, n = 4; *P < .05.

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