Deviant recognition of distinct terminal carbohydrate moieties and specific loss of Galα reactivity in symptomatic PAD. (A) Binary deviation map (P < .05) of IgG immunoprofiles compared with healthy controls based on 2-way analysis of variance and hierarchical clustering analysis with identification of cliques 1 to 4. The rows in this matrix represent the binary antibody-reactivity profiles, and the columns represent the deviation immune profiles for each PAD subgroup. (B) Terminal carbohydrate moieties of epitopes in reactivity clique 2 (nonsignificant deviation) and clique 4 (significant deviation). Numeric occurrence is indicated in parenthesis. Recognition of Galα (C) or GalNAc (D) epitopes within clique 4 (significant deviation). (E) Deviation map based on degree of significance with identification of cliques A through E. (F) Bubble chart displaying terminal glycan structure distribution across cliques A through E. The size key and percentage numbers represent the frequencies.