M2BPGi values and relationship between COI values for M2BPGi and GVHD severity score. (A) COI values for M2BPGi in the healthy control group (n = 20) and in patients who underwent autologous HSCT (n = 11) and allogeneic HSCT (n = 110). The group with allogeneic HSCT consisted of the discovery cohort and validation cohort 1. Samples with autologous HSCT and allogeneic HSCT were collected at day +180. Data are shown as box-and-whisker plots that indicate the 90th and 10th percentiles. Horizontal black lines represent the median values, and circles indicate outliers. P values compare the group with allogeneic HSCT with the healthy control group or with the group with autologous HSCT. The Mann-Whitney U test was used to compare the data. Association between M2BPGi at day +180 and NIH global severity score (B) or liver severity score (C) in the entire cohort. The NIH global severity score and liver severity score were evaluated at sample collection. Data are shown as box-and-whisker plots that indicate the 90th and 10th percentiles. Horizontal black lines represent median values, and circles indicate outliers. The Jonckheere-Terpstra test was used to evaluate the association between M2BPGi and the NIH global severity score or liver severity score.