Perturbed H3K27 methylation in Gata1s mutant erythroid cells. (A) Western blot depicting the levels of H3K27me3 and H3K9me3 at different days of erythroid cell maturation from human CD34 cells. Total H3 and total H4 are shown as loading controls. The intensity of the bands was measured with ImageJ (right). (B) H3K27me3 and H3K9me3 levels were assayed in G1-ER cells at different time points following induction of differentiation by 20 nM β-estradiol. The intensity of the bands was measured with ImageJ (right). (C) Metaplots of H3K27me3 CUT&RUN signal comparing WT and Gata1s E13.5 erythroid cells plotted across a 10-kb window; the y-axis indicates depth per million mapped reads. (Left) R1/R2 population; (right) R3 population. (D) Western blot of the levels of H3K27me3 in fetal liver cells from WT and Gata1s E13.5 embryos.