Loss of the N terminus of GATA1 alters its chromatin occupancy. (A) Venn diagram showing the numbers of binding sites of GATA1 full-length vs GATA1s in E13.5 erythroid cells as determined by CUT&RUN-seq of the endogenous GATA1 proteins. The absence of the N terminus did not alter binding to 79 910 sites (gray), but resulted in gain of GATA1s binding on 576 sites (red) and decreased GATA1s binding on 3268 sites (blue). (B) Integration of CUT&RUN-seq and ATAC-seq data highlights changes in occupancy and accessibility. A total of 329 of the 576 gained sites (red; eg, Fli1 and Mef2c loci) also gained chromatin accessibility, whereas 1844 sites (blue) that lost binding (eg, Lmo2 and Zfpm1 loci) also showed reduced chromatin accessibility. Aggregated binding signals are shown above the heatmaps for gained and lost sites by CUT&RUN-seq and ATAC-seq, respectively. (C) Pie charts showing the distribution of sites that were gained or lost by GATA1s. The rank lists of binding motifs of sites that lost (D) or gained (E) binding of GATA1s.