PN-1 deficiency in mice with F8 deficiency or PN-1 blocking in blood of hemophilia A patients improves clot stability and limits clot lysis. Representative whole blood thromboelastogram (ROTEM) profiles for hemophilic mice (A) or a patient with hemophilia (D). The maximum clot firmness corresponds to the maximum amplitude reached during the test (B,E), and the lysis on time corresponds to the time span from clotting time to the start of significant lysis (C,F). Each dot represents 1 mouse (B-C) or 1 patient (E-F). Values are means ± SEM. A 2-way ANOVA followed by a multiple comparison test was used. *P < .05; **P < .01; ****P < .0001.