PN-1 deficiency enhances thrombin generation in PRP from mice with an F8 deficiency after collagen-induced platelet activation. Representative thrombin generation profiles and thrombin peaks obtained after TF activation (A-B,E-F), or after TF and collagen activation (C-D,G-H) in PRP from F8^−/−, F8^−/−/PN-1^−/− and F8^−/− mice in the presence of 100 µg/mL irrelevant IgG antibody or 100 µg/mL anti-PN-1 antibody. Each dot represents 1 mouse. Values are means ± SEM. A Mann-Whitney test was used for B and D; *P < .05. A non-parametric 1-way ANOVA test followed by a multiple comparison test was used for F and H; *P < .05, **P < .01.