Analysis of paired BM and PB samples in 125 patients. (A) Histogram plot of paired BM and PB RUNX1-RUNX1T1 TLs during therapy and the posttreatment period of the 125 patients, illustrating sample acquisition, interindividual RUNX1-RUNX1T1 TLs, intraindividual correlation of BM and PB TLs, and the MRD course of each patient. Color-coded histograms represent paired RUNX1-RUNX1T1 TLs in BM and PB (y-axis) at the indicated time points (x-axis) for each patient (z-axis). Missing samples are denoted by a corresponding blank area. (B) RUNX1-RUNX1T1 TL kinetics of paired BM and PB samples during therapy and follow-up. (C) Spearman rank correlation of RUNX1-RUNX1T1 TLs of all 680 paired BM and PB samples obtained during therapy and in the posttreatment period. (D) Distribution of paired samples according to their qRT-PCR status (positive/negative) in BM and PB.