Molecular characterization of N5A AMKL. (A) Scatterplots showing pairwise correlation of gene expression values (log FPKM, RNAseq) between N5A AMKL derived from pediatric patients and xenograft models (N5A pAMKL and xAMKL, respectively). For each scatterplot the Pearson correlation coefficients (r) are indicated in mirroring cells. The red diagonal line represents a perfect correlation (r = 1.0). (B) Top 30 genes differentially expressed by at least 10-fold in a sampling of BM cells derived from 2 patients and 4 xenograft models presenting N5A AMKL, as compared with normal CB-CD34⁺ cells (n = 4). Genes with expression values of ≥10 FPKM for all N5A AMKL samples and fold changes ≥10 compared with CB-CD34⁺ samples are displayed (see supplemental Figure 7 for the complete list). The indicated FPKM values are represented by a logarithmic color scale (log₁₀). (C) Expression of HOXA/HOXB cluster and MEIS genes in N5A AMKL samples derived from patients (n = 2) and xenograft models (n = 4) compared with control CB-CD34⁺ cells (n = 4). Values are presented as the mean ± SEM. (D) Top 10 ranked gene sets in the GSEA of genes upregulated in N5A AMKL patients and xenograft models compared with CB-CD34⁺ samples. Enrichment plots for selected gene sets (in red) are depicted in panel E and in supplemental Figure 6.