Identification and characterization of circMYBL2 in FLT3-ITD⁺ AML. (A) Identification of circRNAs having significant differences (P < .01) in FLT3-ITD⁺ AML compared with FLT3-ITD^– AML. (B) Differential expression of circMYBL2 between FLT3-ITD⁺ and FLT3-ITD^– AML patient samples. (C) Structures of the MYBL2 genome and transcript. circMYBL2 is produced by exons 8-9. (D) Identity of the junction point of circMYBL2. (E) RNase R treatment confirmed the circular form of circMYBL2. (F-G) Identification of circMYBL2 cytoplasmic distribution by qRT-PCR analysis and FISH. MTOC1 and MALAT1 were used as the cytoplasmic and nuclear markers, respectively. Cy3 dye and DAPI stain; original magnification ×63. DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.