Clonotypic heterogeneity of skin lesions in MF. (A) The TCF for the skin samples was estimated by the copy-number aberration (CNA) data from the WES. The tumor cell fraction (gray bars) is plotted vs the cumulative frequency of the most abundant TCR-β clonotypes. The frequencies of the clonotypes are represented, with stacked bars representing clonotypes from the most abundant (rank 1) to the least frequent. The ranks of clonotypes are color-coded as in the legend. (B) Correlation between tumor cell fraction and the number of neoplastic clonotypes. Note that the clonotypic heterogeneity is not dependent on the stage of the lesion (tumor vs plaque). The size of the circle is proportional to the percentage of the most dominant (rank 1) TCR-β clonotype. (C) Topological heterogeneity in MF. Venn diagrams illustrating the number of overlapping TCR-β clonotypes across different skin lesions. The location and type of the lesion is plotted for each patient (green circle, plaque; red square, tumor). (D-E) VJ combination diversity of TCR-β clonotypes in MF. The combinations of VJ genes of the neoplastic clonotypes are presented in panels D (MF32) and E (MF16).