Changes in mutant JAK2 (p.V617F) VAF over time. (A) VAF in evaluable samples at baseline, month 12, and end of study. The added P-values highlight a significant difference in median JAK2V617F VAF between CR and NR groups in baseline samples (Wilcoxon test; P = 9.51e-03). (B) JAK2V617F VAF waterfall plot. The y-axis indicates the absolute change of JAK2V617F VAF from baseline to last sample. Each bar represents a patient, highlighted by the best clinical response (as described in the color key). Analysis of the number of patients with increased and decreased VAF between groups indicates that the chance of achieving CR is 15 times higher for patients with a reduced JAK2V617F allele burden (Fisher’s exact test; P = 9.071e-06; OR, 15).