RNase treatment decreases perfusion recovery and vessel growth. (A-B) Laser Doppler perfusion measurements (upper panels) and corresponding flux images (lower panels) of mice treated as indicated. The perfusion was calculated using occluded/sham (right to left) ratios before the surgical procedure, immediately after the surgical procedure, and at days 3 and 7 after surgery (n = 6). Representative flux images are shown for days 3 and 7. (C) The dot plots show the inner luminal diameter of mice treated with saline, RNase A, or recombinant inactive or active human RNase1 7 days after the surgical procedure (n = 6 per group). Data are mean ± SEM. The dashed horizontal line indicates the mean sham value. (D) Representative photomicrographs of Giemsa-stained tissue samples. Scale bars, 20 µm. *^,#P < .05; *saline vs RNase A, ^#DNase vs RNase A, *saline vs active human RNase1, ^#inactive human RNase1 vs active human RNase1, 2-way analysis of variance (ANOVA) with the Bonferroni multiple-comparison test (A-B) and 1-way ANOVA with the Bonferroni multiple-comparison test (C).