The role of necdin in adult hematopoiesis. (A) Necdin-null (Ndn-null) fetal liver cells (CD45.2) normally repopulated lethally irradiated recipient mice (CD45.1). The frequency of donor-derived cells (CD45.2) in peripheral blood was measured monthly by flow cytometry at 4 time points. SDs are shown (left panel). Right panels show the data from flow cytometry 16 weeks after transplantation. (B) Recipient mice receiving necdin-null fetal liver cells (1 × 10⁶) showed normal multilineage reconstituting activity, as assessed by the percentage of donor-derived myeloid cells (bottom left panel), B cells (top right panel), and T cells (top left panel) 16 weeks after transplantation using flow cytometry. (C) The frequency of HSCs (Lin⁻Sca-1⁺c-kit⁺CD48⁻CD150⁺ cells) in the BM of mice reconstituted with wild-type (WT) or necdin-null fetal liver cells was measured by flow cytometric analysis using SLAM cell-surface markers. Data shown are the mean percentage of HSCs (± SD; P = .1, n = 14). (D) Analysis of the common myeloid progenitor (CMP), granulocyte monocyte progenitor (GMP), and megakaryocyte erythrocyte progenitor (MEP) compartments showed comparable frequencies for those transplanted mice that received wild-type versus necdin-null fetal liver cells (P = .44, P = .41, P = .33, respectively, n = 6).