Effects on specific inhibition of S1PR1 in Ly3 ABC-DLBCL cell line. (A) Cell proliferation and apoptosis were assessed 24 or 48 hours after culturing Ly3 tumor cells with or without S1PR1 shRNA knockdown. Data represent 3 independent experiments. ***P < .001. **P < .01. *P < .05. (B) Real-time PCR showing differences in mRNA expression levels of STAT3 downstream genes in control and S1PR1 shRNA lentivirus-transduced Ly3 tumor cells. ***P < .001. **P < .01. *P < .05. Each sample was examined by triplicates. (C) Western blot analysis of protein expression levels of STAT3 downstream genes in both control and S1PR1 shRNA-expressing Ly3 tumor cells. Data represent 3 independent experiments.