Schematic representation of iNOS-induced metabolic changes in DCs after TLR ligation. Unstimulated DCs can use both glycolysis and mitochondria for their bioenergetic and metabolic needs; however, TLR ligation induces iNOS expression and NO production that in an autocrine fashion inhibits the ETC and mitochondrial function. As a consequence, TLR-activated DCs enhance their glycolytic rate to prevent bioenergetic collapse and cell death.