Ly6G Ab 1A8 inhibits LTB₄-induced neutrophil migration. (A) LTB4 induced-migration of CD45⁺Gr-1⁺CD11b^hi neutrophils in B6 BM after isotype or anti-Ly6G preincubation (10 μg/mL for 10 minutes) expressed as the percentage of migration in the absence of chemoattractant. Data presented are from 4 independent experiments. (B) The isotype-matched binding control anti-Ly6B.2 (10 μg for 10 minutes) did not affect neutrophil migration toward LTB4. (C) Migration assay using BM deficient in FcγRII, demonstrating preserved inhibition of migration. (D) Effect of anti-Ly6G Fab fragments (10 μg/mL for 10 minutes) on neutrophil migration. In panels B-D, data are representative of 2 independent experiments with similar results. *P < .05; **P < .01.