Ab ligation of Ly6G at low doses does not induce neutrophil cell death in vitro and in vivo. (A) Cells from whole BM and TG-stimulated peritoneum were cultured with isotype or anti-Ly6G at 10 μg/mL, and neutrophil apoptosis was assessed at the indicated time points by annexin V. (B) Anti-Ly6G (5 μg) was administered intraperitoneally to mice as per the arthritis protocol and TG was instilled intraperitoneally at the equivalent of day 4 of arthritis. Peritoneal cells were harvested at 4 hours by lavage and neutrophils stained for annexin V and the percentage of live/dead cells. Negative and positive controls for cell apoptosis/death were BM cells cultured in the absence or presence of the apoptosis inducer staurosporine (3μM) for 4 hours. Results are representative of 2 independent experiments.