Figure 5
Enhanced cellular senescence in lymphomas and premalignant B-lymphoid cells from Eμ-myc/E6AP+/− mice. (A) Senescence-associated β-galactosidase (SA-β-gal) activity was assayed in lymph nodes and spleen sections at manifestation of lymphoma in Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc mice. Quantification of SA-β-gal positive cells: > 400 cells/tumor, n = 4/genotype were counted in 4 randomly selected fields; *P < .001,**P < .001. Values represent mean ± SD. Magnification ×200. (B) SA-β-gal activity was assayed in cytospins of premalignant B cells isolated from BM of 4-week-old Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc mice. Quantification of SA-β-gal positive cells: > 400 cells/mice, n = 3/genotype were counted in 4 randomly selected fields; P < .001. Values represent means ± SD. Magnification ×200. (C) The levels of H3K9me3 in Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc lymphomas (n = 10/genotype) were measured by FACS analysis after staining with anti-H3K9me3 antibodies. Representative histograms are shown. Summary of results is shown in supplemental Figure 5. (D) The levels of H3K9me3 in BM derived premalignant B lymphoid cells of 4-week-old Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc mice were measured by flow cytometry after staining with anti-H3K9me3 antibodies (n = 3/genotype). Representative histograms are shown.

Enhanced cellular senescence in lymphomas and premalignant B-lymphoid cells from Eμ-myc/E6AP+/− mice. (A) Senescence-associated β-galactosidase (SA-β-gal) activity was assayed in lymph nodes and spleen sections at manifestation of lymphoma in Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc mice. Quantification of SA-β-gal positive cells: > 400 cells/tumor, n = 4/genotype were counted in 4 randomly selected fields; *P < .001,**P < .001. Values represent mean ± SD. Magnification ×200. (B) SA-β-gal activity was assayed in cytospins of premalignant B cells isolated from BM of 4-week-old Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc mice. Quantification of SA-β-gal positive cells: > 400 cells/mice, n = 3/genotype were counted in 4 randomly selected fields; P < .001. Values represent means ± SD. Magnification ×200. (C) The levels of H3K9me3 in Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc lymphomas (n = 10/genotype) were measured by FACS analysis after staining with anti-H3K9me3 antibodies. Representative histograms are shown. Summary of results is shown in supplemental Figure 5. (D) The levels of H3K9me3 in BM derived premalignant B lymphoid cells of 4-week-old Eμ-myc/E6AP+/− and control (E6AP+/+) Eμ-myc mice were measured by flow cytometry after staining with anti-H3K9me3 antibodies (n = 3/genotype). Representative histograms are shown.

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